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ImmunoRatio is a free web application for automated image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67 immunostained tissue sections.

The application:

  1. segments diaminobenzidine-stained (DAB) and hematoxylin-stained nuclei regions from the user-submitted image,
  2. calculates the percentage of DAB-stained nuclear area over total nuclear area (=labeling index), and
  3. generates a pseudo-colored result image matching the area segmentation (see the example on right side).

ImmunoRatio is also available as a plugin version for the ImageJ image analysis software. For downloads and more details, please see the plugin webpage.

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